Formate dehydrogenase, in the process of anaerobic fermentation of glycerol to produce 1,3-propanediol, needs to consume reducing equivalents of NADH, and the effective supply of NADH determines the yield and yield of 1,3-propanediol. The gene fdh encoding formate dehydrogenase was cloned from the Candidaboidinii genome by PCR method, and the fdh gene fragment was inserted into the vector pMAL^TM-p2X to construct the expression vector pMAL^TM-p2X-fdh, and transferred into 1,3-propanediol producing bacteria KlebsiellapneumoniaeYMU2, the recombinant strain KlebsiellapneumoniaeF-1 was obtained.